The
Johns Hopkins Hospital
Sample Molecular Epidemiology Laboratory Report
|
Date
|
5/27/99
|
|
Method
|
PFGE
|
|
% Agarose
|
1%
|
|
Location
|
ICU-A
|
|
Enzyme
|
SmaI
|
|
Buffer
|
.5X
|
|
Organism
|
MRSA
|
|
Pulse
Time
|
5 to
40 sec
|
|
Tech
Initials
|
sh
|
|
Gel
No.
|
1
|
|
Run
Time
|
24 hours
|
|
Reviewer
Initials
|
wgm
|
Description
of Isolates:
|
Lane
|
Name
|
Date
|
Specimen
No.
|
Body
Source
|
Comment
|
|
1
|
Molec.
Wt. Marker
|
|
|
|
E.
faecalis OGIRF
|
|
2
|
Patient
1
|
10/19/96
|
|
endoctracheal
|
|
|
3
|
Patient
2
|
6/20/97
|
|
nares
|
|
|
4
|
Patient
3
|
6/20/97
|
|
nares
|
|
|
5
|
Patient
4
|
5/27/97
|
|
blood
|
|
|
6
|
Patient
5
|
6/20/97
|
|
nares
|
|
|
7
|
Patient
6
|
6/17/97
|
|
blood
|
|
|
8
|
HCW 1
|
6/26/97
|
|
nares
|
|
|
9
|
HCW 2
|
6/30/97
|
|
nares
|
|
|
10
|
Molec.
Wt. Marker
|
|
|
|
E.
faecalis OGIRF
|
|
|
Epidemiologic
Question:
|
Is the MRSA
from Patient 1, isolated 10/96 at Hospital A, related
to a cluster of MRSA cases (Patients 2 to 6) from
May and June of 1997? Two health care workers (HCW)
were colonized with MRSA at the time the cluster was
discovered. Are the isolates from HCW1 and HCW2 related
to patient strains?
|
|
|
Interpretation:
|
MRSA isolated
in 1997 from Patients 2,3,4,5, and 6 are indistinguishable.
The MRSA from HCW2 has the same fingerprint pattern
as the clustered patients. These six cases are the
same strain type ICU-A. Patient 1 has a closely related
isolate, subtype ICU-A1. HCW1 also has a strain closely
related to the outbreak, ICU-A2.
|
|
|
